Effect of bisphosphonate on osteoclast differentiation and tartrate-resistant acid phosphatase

doi:10.3969/j.issn.2095-4344.2014.38.001

Abstract

Abstract:

BACKGROUND: Tartrate-resistant acid phosphatase is a specific marker for osteoclast differentiation and bone resorption, which is a sign of osteoclast maturity.
OBJECTIVE: To study the effect of alendronate on tartrate-resistant acid phosphatase related to osteoclast differentiation and bone resorption.
METHODS: Osteoclasts were cultured by mouse monocyte-macrophage cell line-RAW264.7. The cells were divided into two groups: control group, treated with 100 μg/L receptor activator of nuclear factor κB ligand factor; alendronate group, treated with 100 μg/L receptor activator of nuclear factor κB ligand factor+10-7 mol/L alendronate. Osteoclastogenesis and resorption function of osteoclasts were examined at 7 days of culture. Gene expression of tartrate-resistant acid phosphatase was detected by immunofluorescence method. Western blot assay was used to detect protein expression of tartrate-resistant acid phosphatase.
RESULTS AND CONCLUSION: Tartrate-resistant acid phosphatase positive multinuclear cells were observed and resorption lacunae formed in two groups. Control group showed the higher number of tartrate-resistant acid phosphatase positive multinuclear cells and larger size of resorption lacunae than the alendronate group (P < 0.01). Immunofluorescence showed expression of tartrate-resistant acid phosphatase was higher in the control group than the alendronate group (P < 0.01); furthermore, the protein expression of tartrate-resistant acid phosphatase was also lower in the alendronate group than the control group (P < 0.01). These findings indicate that bisphosphonates could strongly inhibit osteoclastogenesis and its resorption function by inhibiting protein expression of tartrate-resistant acid phosphatase.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: tissue engineering, diphosphonates, osteoclasts, osteoporosis

CLC Number:

R318

Dong Wei, Feng Xiao-jie, Liang Yong-qiang, Deng Jiu-peng, Wen Li-ming, Qi Meng-chun. Effect of bisphosphonate on osteoclast differentiation and tartrate-resistant acid phosphatase[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(38): 6069-6073.

Figures/Tables 2

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